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KMID : 0811720010050040323
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Korean Journal of Physiology & Pharmacology
2001 Volume.5 No. 4 p.323 ~ p.332
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Identification and characterization of Shiga toxin-producing Escherichia coli isolated from diarrhea in calves
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Yong-Kyoo Shin/Hyang Mi Lee
Yoon Young Jang/Jin Ho Song/Kwang Joon Kim/In Ja Lim/Yong Kyoo Shin
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Abstract
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Shiga toxin (stx) producing Escherichia coli (STEC) causes various clinical signs in animal and human. In this study, 255 fecal samples from calves showing diarrhea were collected from cattle farms in Chonnam province during the period from January 2005 to July 2005. Twenty six STEC (10%) were isolated from 255 fecal samples by PCR. The isolates displayed three different stx combinations (stx1 [69%], stx1 and stx2 [15%], and stx2 [38%]). The isolates were further studied for virulence associated genes and antimicrobial resistance to define the virulence properties. Intimin (eaeA), enterohemolysin (hlyA), and lipopolysaccharide (rfbE) virulence genes were detected in 6 (23%), 7 (26%), and 1 (3.8%) of the isolates, respectively, by PCR. One isolate possessing rfbE gene was typed as E. coli 0157 : H7 by agglutination test with O and H antisera. All 26 isolates showed susceptibility to amikacin (100%) and the majority of isolates showed high susceptibility to gentamicin (88.5%) and chloramphenicol (73.1%). But all isolates were resistant to penicillin. These results may provide the basic knowledge to establish strategies for the treatment and prevention of enteric disease in calves.
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KEYWORD
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Rutin, Ischemia, Reperfusion,
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